Changes in Molecular Forms of Rat Hepatic Glutathione S-Transferase during Chemical Hepatocarcinogenesis1

نویسندگان

  • Akio Kitahara
  • Kimihiko Satoh
  • Koya Nishimura
  • Takashi Ishikawa
  • Kazuo Ruike
  • Kiyomi Sato
  • Hiroyuki Tsuda
  • Nobuyuki Ito
چکیده

Changes in molecular forms of hepatic cytosolic glutathione S-transferases (GST) during rat chemical hepatocarcinogenesis were investigated. GST activities toward 1-chloro-2,4-dinitrobenzene and 1,2-dichloro-4-nitrobenzene increased with the in creased area of 7-glutamyltranspeptidase-positive foci and hyperplastic nodules induced by diethylnitrosamine followed by 2acetylaminofluorene plus hepatectomy. Among GSTs with high activities toward 1,2-dichloro-4-nitrobenzene, which were sepa rated by carboxymethyl Sephadex column chromatography, the activity of GST-A (YbYb) markedly increased with increased activity towards 1,2-dichloro-4-nitrobenzene in livers bearing foci and nodules and in isolated nodules and hepatomas, while activities of GST-C (YbYb') and -D (Yb'Yb') changed little. It was demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and two-dimensional gel electrophoresis that Yb as well as Ya, a subunit of ligandin (YaYa) and GST-B (YaYc), increased in livers bearing foci and nodules, while Yc as well as Yb' changed little. A new placental GST form (GST-P), which has a subunit molecular weight of 21,500 or 26,000, according to the marker proteins used, and neutral pis of 6.8 and 6.3, is immunologically different from any form of basic GSTs and is very low in normal liver; also, it was markedly induced in livers bearing foci and nodules and in well-differentiated hepatomas but not by short-term administration of drugs such as 2-acetylaminofluorene, in contrast to GST-A and ligandin. These re sults indicate that GST-A and more especially GST-P could be new preneoplastic marker enzymes for chemical hepatocarcino genesis.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Changes in molecular forms of rat hepatic glutathione S-transferase during chemical hepatocarcinogenesis.

Changes in molecular forms of hepatic cytosolic glutathione S-transferases (GST) during rat chemical hepatocarcinogenesis were investigated. GST activities toward 1-chloro-2,4-dinitrobenzene and 1,2-dichloro-4-nitrobenzene increased with the increased area of gamma-glutamyltranspeptidase-positive foci and hyperplastic nodules induced by diethylnitrosamine followed by 2-acetylaminofluorene plus ...

متن کامل

CHANGES IN GLUTATHIONE S-TRANSFERASE ACTIVITY AND ZEARALENONE CONTENT IN SUSCEPTIBLE AND TOLERANT WHEAT HEADS INOCULATED WITH FUSARIUM GRAMINEARUM, THE CAUSAL AGENT OF FUSARIUM HEAD SCAB

Glutathione S-transferase (GST) activity pattern was determined in tolerant (cv. Sumai#3) and susceptible (cv. Falat) wheat heads inoculated with Fusarium graminearum, the causal agent of head scab disease (FHB), during various developmental stages. GST specific activity exhibited a transient pattern in Sumai#3 reaching a maximum level at the milk stage and declining thereafter. GST level in Su...

متن کامل

Antioxidant properties and Glutathione S-transferases inhibitory activity of Alchornea cordifolia leaf extract in Acetaminophen toxicity

Paracetamol (acetaminophen, PCM) is a widely used over-the-counter analgesic and antipyretic drug. Intake of a large dose of PCM may result in severe hepatic necrosis. Oxidative stress is mediated by oxidative capacities of the PCM metabolite (N-acetyl-para-benzo quinoneimine, NAPQI), which covalently binds to proteins and other macromolecules to cause cellular damage. At low doses, NAPQI is ...

متن کامل

Correlation of changes in natural killer cell activity and glutathione S-transferase placental form positive hepatocytes in diethylnitrosamine-induced rat hepatocarcinogenesis.

To evaluate the induction of preneoplastic hepatic foci in relation to natural killer cell (NK) activity, we sequentially analyzed glutathione S-transferase placental form positive (GST-P+) hepatocytes and NK activity during diethylnitrosamine (DEN) and phenobarbital (PB)-induced hepatocarcinogenesis in Sprague-Dawley rats. Previous studies have shown that NK activity can modulate the carcinoge...

متن کامل

Elevation of extrahepatic glutathione S-transferase and epoxide hydratase activities by 2(3)-tert-butyl-4-hydroxyanisole.

Investigations in these and other laboratories have estab lished that administration of 2(3)-tert-butyl-4-hydroxyanisole (BHA) to rodents: (a) protects a variety of target tissues against the production of tumors by a wide range of chemical carcin ogens; (b) reduces the levels of mutagenic metabolites pro duced from benzo(a)pyrene and numerous therapeutic agents in viva; (c) elevates the hepati...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 1984